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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has become observed in lots of tumors, which ends up in chromosome instability, and so may contribute to tumorigenesis. This protein was located to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a important function in endorsing mobile-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are already described. Two supplemental splice variants have been described but comprehensive nucleotide sequence info is not really nevertheless accessible.
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Blocking peptides are peptides that bind specially to the focus on antibody and block antibody binding. These peptide usually includes the epitope identified by the antibody.
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This tutorial outlines even more achievable optimization of cellular and immunoassay parameters to ensure the very best benefits are...
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This manual outlines additional possible optimization of cellular and immunoassay parameters to make sure the best possible outcomes are...
Antibodies bound to the blocking peptide not bind for the epitope within the goal protein. By comparing the staining from the blocked antibody versus the antibody by itself, you can see which staining is distinct.
Focus on Details Cyclin E belongs for the extremely conserved cyclin family members, whose members are characterised by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which add to your temporal coordination of every mitotic event. Cyclin E sorts a complex with and features to be a regulatory subunit of CDK2, whose action is necessary for cell cycle G1/S changeover. This protein accumulates at the G1-S stage boundary and it is degraded as cells progress via S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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